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Methods, Models, Tools, and Databases for Water Research

Methods

Drinking Water Treatment and Analysis

  • Biological Treatment Process for the Removal of Ammonia from a Small Drinking Water System from Pilot- to Full-Scale 
    This engineering design and operation document describes the operation and treatment effectiveness of a small, full-scale biological ammonia removal drinking water treatment plant. The treatment plant engineering design criteria and operating conditions are presented, and development of the project from pilot- to full-scale is discussed. Lastly, lessons learned from the project, and future considerations when designing and operating biological treatment systems for ammonia reduction are presented.
  • Drinking Water Methods
    Residues of unregulated contaminants may be present in drinking water, however little is known about their prevalence in the environment. To protect human health, EPA scientists have developed a number of methods to detect unregulated contaminants.
  • Microbiological Methods and Online Publications (Bacteria, Protozoans, Viruses, and Coliphage)
    EPA scientists have developed a variety of methods to detect, characterize and study bacteria, protozoans, viruses and coliphage. These methods are used by EPA and state and regional water quality managers to protect human health by keeping drinking water and recreational water safe.
  • Removal of Fluoride from Drinking Water Supplies by Activated Alumina
    This design manual is an update to the 1984 version by the same title. It provides an in-depth presentation of the steps required to design and operate a fluoride removal plant using activated alumina, which is a reliable and cost-effective process for treating excess fluoride from drinking water supplies.
  • Standard Test Methods for Perfluorinated Compounds (PFC)
    Method 537 is currently used only for drinking water samples. Although nationally approved methods for measuring PFCs in non-drinking water samples are not yet available, the following consensus organization methods are provided by the American Society for Testing and Materials (ASTM): (ASTM D7968) Standard Test Method for Determination of Perfluorinated Compounds in Soil by Liquid Chromatography Tandem Mass Spectrometry, and (ASTM D7979) Standard Test Method for Determination of Perfluorinated Compounds in Water, Sludge, Influent, Effluent and Wastewater by Liquid Chromatography Tandem Mass Spectrometry. 

Environmental Waters

  • Microbiological Methods and Online Publications (Bacteria, Protozoans, Viruses, and Coliphage)
    EPA scientists have developed a variety of methods to detect, characterize and study bacteria, protozoans, viruses and coliphage. These methods are used by EPA and state and regional water quality managers to protect human health by keeping drinking water and recreational water safe.
  • Biological Methods and Manual Development
    EPA's research in stream and source monitoring indicators includes fish, macroinvertebrates, periphyton, zooplankton, functional ecosystem indicators, water and sediment toxicity, and fish tissue contaminants. EPA exposure scientists regularly prepare and update field and laboratory protocol and methods manuals. They also provide technical assistance to EPA regions, program offices and states on the implementation and interpretation of these manuals. This website lists currently available manuals and protocols.
  • Standard of Practice for Determination of Nonylphenol Polyethoxylates (NPnEO, 3 ≤ n ≤ 18) and Octylphenol Polyethoxylates (OPnEO, 2 ≤ n ≤ 12) in Environmental Waters EXIT
    Nonylphenol (NP) and octylphenol (OP) have been shown to have toxic effects in aquatic organisms. The prominent source of NP and OP is from common commercial surfactants, which are longer chain alkylphenol ethoxylates (APEOs). This practice screens for the longer chain APEOs which may enter sewage treatment plants at elevated levels and may cause violations of permitted discharge concentration of NP. It covers determination of NP and OP polyethoxylates in water by Single Reaction Monitoring Liquid Chromatography/ Tandem Mass Spectrometry using direct injection liquid chromatography and detected with tandem mass spectrometry detection. 
  • Standard Test Method for Bisphenol A in Environmental Waters Exit
    The environmental source of BPA is predominantly from the decomposition of polycarbonate plastics and resins, which are used in a wide range of commercial products. BPA has been reported to have adverse effects in aquatic organisms and may be released into environmental waters directly at trace levels through landfill leachate and POTW effluents. This method has been investigated for use with surface water and secondary and tertiary POTW effluent samples therefore, it is applicable to these matrices only.

Wastewater and Water Reuse

  • Guidelines for Water Reuse (PDF)
    This manual provides comprehensive, up-to-date national guidance on water reuse regulations and program planning in support of regulations and guidelines developed by states, tribes, and other authorities.
  • Standard of Practice for Determination of Nonylphenol Polyethoxylates (NPnEO, 3 ≤ n ≤ 18) and Octylphenol Polyethoxylates (OPnEO, 2 ≤ n ≤ 12) in Environmental Waters EXIT
    Nonylphenol (NP) and octylphenol (OP) have been shown to have toxic effects in aquatic organisms. The prominent source of NP and OP is from common commercial surfactants, which are longer chain alkylphenol ethoxylates (APEOs). This practice screens for the longer chain APEOs which may enter sewage treatment plants at elevated levels and may cause violations of permitted discharge concentration of NP. It covers determination of NP and OP polyethoxylates in water by Single Reaction Monitoring Liquid Chromatography/ Tandem Mass Spectrometry using direct injection liquid chromatography and detected with tandem mass spectrometry detection.