Focus up and down through the organism a number of times while adjusting the prism to observe the internal morphology completely. The prism should be adjusted through the mid-point (or darkest) point to observe the structures with light cast from multiple directions.
Adjusting the amount of light using the stage diaphragm and light source rheostat helps by altering the contrasting appearance of the internal features of the cell.
Use the location of the DAPI stained nuclei to help find the corresponding nuclei on DIC.
Sometimes the median bodies will be highlighted by DAPI. Use this to help locate the structures on DIC.
When using DIC microscopy, the background color varies depending on the filters in place. Gray to blue is the typical color used.
Always use a clean microscope and the minimum amount of non-fluorescing immersion oil necessary to ensure optimal contrast and resolution.