All size measurements must be determined using 1000x magnification and reported to the nearest 0.5 micrometers (µm).
- Superimpose the ocular micrometer over the cyst.
- Place the left edge of the widest point of the cyst on a line of the micrometer.
- Count the number of units to the right edge of the cyst.
- Convert the number of units counted to µm using the microscope conversion factor previously determined (see section 10.3.5 of June 2003 Draft of Method 1622/1623: Calibration of an ocular micrometer).
- Repeat for the longest point of the cyst.