We develop antimicrobial testing methods and standard operating procedures to measure the effectiveness of hard surface disinfectants against:
- Staphylococcus aureus.
- Pseudomonas aeruginosa.
- Salmonella choleraesuis.
- Mycobacterium bovis (BCG).
- Clostridium difficile.
Methods are available for the following types of antimicrobial formulations:
Our standard operating procedures are strict interpretations of AOAC International Exit and ASTM International standard methods. Exit
||Biosafety in the Laboratory
||Tracking of Test Microorganisms
||Screening of Polished Stainless Steel Cylinders, Porcelain Cylinders and Glass Slide Carriers Used in Disinfectant Efficacy Testing
||AOAC Use Dilution Method for Testing Disinfectants
||Germicidal Spray Products as Disinfectants (GSPT): Testing of Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella enterica
||Tuberculocidal Activity of Disinfectants: In Vitro Test for Determining Tuberculocidal Activity
||Disinfectant Towelette Test: Testing of Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella enterica
||Media and Reagents Preparation and Quality Evaluation
||Neutralization Confirmation Assay for Disinfectant Products Tested against Mycobacterium bovis (BCG)
||Handling Spills of Biohazardous Materials
||Sporicidal Activity of Disinfectants Test (Bacillus × Porcelain component only)
||Quantitative Suspension Test Method for Determining Tuberculocidal Efficacy of Disinfectants against Mycobacterium bovis (BCG)
||Neutralization Confirmation Procedures for the AOAC Use-dilution method (UDM), the AOAC Germicidal Spray Products as Disinfectants Test (GSPT) and the Disinfectant Towelette Test (DTT)
||Quantitative Three Step Method for Measuring the Efficacy of Liquid Sporicides against Spores of Bacillus subtilis on Hard Non-porous and Porous Surfaces
||Disinfectant Product Preparation and Sampling Procedures
||Disinfectant Towelette Test: Testing of Mycobacterium bovis (BCG)
||Testing of Mycobacterium bovis (BCG) Using the Germicidal Spray Products as Disinfectants Test
||Neutralization of Microbicidal Activity using the OECD Quantitative Method for Evaluating Bactericidal Activity of Microbicides Used on Hard, Non-Porous Surfaces
||Germicidal and Detergent Sanitizing Action of Disinfectants Test
||Production of Spores of Clostridium difficile for Use in the Efficacy Evaluation of Antimicrobial Agents
||Preparation of hard water and other diluents for antimicrobial products
||Quantitative Disk Carrier Test Method (QCT-2) Modified for Testing Antimicrobial Products Against Spores of Clostridium difficile (ATCC 43598) on Inanimate, Hard, Non-porous Surfaces
||Quantitative Petri Plate Method (QPM) for Determining the Effectiveness of Antimicrobial Towelettes Against Spores of Clostridium difficile (ATCC 43598) on Inanimate, Hard Non-porous Surfaces
||Quantitative Petri Plate Method (QPM) for Determining the Effectiveness of Antimicrobial Towelettes Against Vegetative Bacteria on Inanimate, Hard, Non-porous Surfaces
The following standard operating procedures are pertinent to specific equipment and practices used in the EPA pesticide program's Microbiology Laboratory. The SOPs describe the laboratory's standard practices for:
- Calibrating equipment (EQ).
- Performing quality control procedures (QC).
- Administrative procedures (ADM).
- Maintaining chain of custody of samples (COC).
- Assuring quality management practices (QA).
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