Documents on NPDES WET methodology.
You will need Adobe Reader to view some of the files on this page. See EPA’s About PDF page to learn more.- Clarifications Regarding Toxicity Reduction and Identification Evaluations in the NPDES Program (PDF)(22 pp, 84 K)
- Clarifications Regarding Toxicity Reduction and Identification Evaluations in the NPDES Program Memo (PDF)(2 pp, 227 K)
- Clarifications Regarding Whole Effluent Toxicity Test Methods Recently Published at 40 CFR Part 136 and Guidance on Implementation of Whole Effluent Toxicity in Permits (PDF)(8 pp, 755 K)
- Errata for the Effluent and Receiving Water Toxicity Testing Manuals (PDF)(17 pp, 60 K)
- Generalized Methodology for Conducting Industrial Toxicity Reduction Evaluations (TREs) (PDF)(107 pp, 1 MB)
- Methods for Aquatic Toxicity Identification Evaluations: Phase I Toxicity Characterization Procedures Second Edition (PDF)(87 pp, 2 MB)
- Methods for Aquatic Toxicity Identification Evaluations: Phase II Toxicity Identification Procedures for Samples Exhibiting Acute and Chronic Toxicity (PDF)(71 pp, 996 K)
- Methods for Aquatic Toxicity Identification Evaluations: Phase III Toxicity Confirmation Procedures for Samples Exhibiting Acute and Chronic Toxicity (PDF)(32 pp, 402 K)
- Toxicity Identification Evaluation: Characterization of Chronically Toxic Effluents, Phase I (PDF)(59 pp, 6 MB)