EPA Region 9 Laboratory Standard Operating Procedures
Note: To obtain a copy of a specific standard operating procedure (SOP), please contact Lucrina Jones (Jones.Lucrina@epa.gov) at (510) 412 - 2321.
| Category | SOP# | SOP TITLE |
|---|---|---|
| Administrative: Purchasing, Shipping,/Receiving & Lab Certification | 010 | Guidance for use of EPA Purchase Cards |
| Administrative: Purchasing, Shipping,/Receiving & Lab Certification | 020 | Shipping and Receiving of Coolers, Supplies and Equipment |
| Administrative: Purchasing, Shipping,/Receiving & Lab Certification | 030 | Sample Coordination by the RSCC |
| Administrative: Purchasing, Shipping,/Receiving & Lab Certification | 050 | State Drinking Water Laboratory Certification Program Oversight |
| Sample Control | 110 | Sample Receipt, Login, and Storage |
| Sample Control | 112 | Sample Receiving in the Absence of the Sample Custodian |
| Sample Control | 115 | Chemical Preservation upon Receipt at the Laboratory |
| Sample Control | 125 | Aqueous Sample Disposal |
| Sample Control | 130 | Glassware Cleaning Procedures |
| Sample Control | 150 | Soil and Sediment Homoginization |
| Chemistry: Organic Sample and Standards Preparation | 205 | Preparation of Organic-free Method Blank Water |
| Chemistry: Organic Sample and Standards Preparation | 239 | Recycling Used Methylene Chloride |
| Chemistry: Organic Sample and Standards Preparation | 260 | Gel-Permeation Chromatography (GPC) Clean-up |
| Chemistry: Organic Sample and Standards Preparation | 275 | Extraction of Water Samples using CLLE |
| Chemistry: Organic Sample and Standards Preparation | 285 | Soxhlet Extraction of Solid Samples |
| Chemistry: Organic Sample and Standards Preparation | 290 | Extraction of Soil Samples Using PFE (Pressurized Fluid Extraction - ASE) |
| Chemistry: Organic Sample and Standards Preparation | 295 | Waste Dilution |
| Chemistry: Organic Sample Analysis | 305 | Volatile Organics Analysis |
| Chemistry: Organic Sample Analysis | 311 | Analysis of Volatile Organic Compounds in Air and Soil Vapor |
| Chemistry: Organic Sample Analysis | 312 | SUMMA Canister Leak Check and Cleaning |
| Chemistry: Organic Sample Analysis | 314 | TO15 SIM-Analysis of Volatile Organic Compounds in Air |
| Chemistry: Organic Sample Analysis | 315 | Semivolatile Organics Analysis |
| Chemistry: Organic Sample Analysis | 325 | Determination of Dissolved Gases in Water |
| Chemistry: Organic Sample Analysis | 330 | Pesticides & PCB (Water, Sediment, Soil) by GC |
| Chemistry: Organic Sample Analysis | 335 | Polychlorinated Biphenyls (PCBs) by GC |
| Chemistry: Organic Sample Analysis | 350 | Analysis of EDB and DBCP in Water by Micro-extraction and GC |
| Chemistry: Organic Sample Analysis | 353 | Low-level Volatiles |
| Chemistry: Organic Sample Analysis | 354 | Volatile Organic Analysis in Water |
| Chemistry: Organic Sample Analysis | 375 | Low-level Semi-Volatile Organics Analysis |
| Chemistry: Organic Sample Analysis | 380 | Purgeable Aromatics and Hydrocarbons by GC PID/FID |
| Chemistry: Organic Sample Analysis | 385 | TPH/DRO Analysis by GC/FID |
| Chemistry: Inorganic Sample and Standards Preparation | 250 | TCLP Extraction |
| Chemistry: Inorganic Sample and Standards Preparation | 252 | California Waste Extraction Test (WET) |
| Chemistry: Inorganic Sample and Standards Preparation | 254 | SPLP Extraction |
| Chemistry: Inorganic Sample and Standards Preparation | 256 | Bioaccessibility Extraction |
| Chemistry: Inorganic Sample and Standards Preparation | 401 | Nepholometric Turbidity Measurement |
| Chemistry: Inorganic Sample and Standards Preparation | 403 | Aqueous Sample Preparation for ICP-AES and ICP-MS |
| Chemistry: Inorganic Sample and Standards Preparation | 405 | Preparation of Solid Samples for Metals Analysis |
| Chemistry: Inorganic Sample and Standards Preparation | 407 | Preparation of Leachate |
| Chemistry: Inorganic Sample and Standards Preparation | 409 | Digestion for Lead on Filters |
| Chemistry: Inorganic Sample and Standards Preparation | 420 | Microwave Digestion of Solid Samples |
| Chemistry: Inorganic Sample and Standards Preparation | 430 | Tissue Preparation and Homogenization |
| Chemistry: Inorganic Sample Analysis | 460 | Percent Solids Determination |
| Chemistry: Inorganic Sample Analysis | 461 | Total Dissolved Solids |
| Chemistry: Inorganic Sample Analysis | 462 | Total Suspended Solids |
| Chemistry: Inorganic Sample Analysis | 463 | Settleable Solids |
| Chemistry: Inorganic Sample Analysis | 464 | Suspended Sediment Concentration |
| Chemistry: Inorganic Sample Analysis | 475 | Flash Point by Pensky-Martens Closed Tester |
| Chemistry: Inorganic Sample Analysis | 503 | Analysis of Solids by ICP |
| Chemistry: Inorganic Sample Analysis | 505 | Determination of Trace Metals in Water by ICP-AES |
| Chemistry: Inorganic Sample Analysis | 507 | Trace Metals by ICP-MS |
| Chemistry: Inorganic Sample Analysis | 509 | Determination of Lead on Filters by ICP-MS |
| Chemistry: Inorganic Sample Analysis | 515 | Mercury Analysis by Cold Vapor Atomic Absorption |
| Chemistry: Inorganic Sample Analysis | 517 | Analysis of Solid Samples for Mercury |
| Chemistry: Inorganic Sample Analysis | 525 | Hexavalent Chromium in Water by Ion Chromatography |
| Chemistry: Inorganic Sample Analysis | 530 | Anions by Ion Chromatography |
| Chemistry: Inorganic Sample Analysis | 531 | Analysis of Perchlorate by Ion Chromatography |
| Chemistry: Inorganic Sample Analysis | 532 | Cyanide Analysis |
| Chemistry: Inorganic Sample Analysis | 535 | Mercury in Solids by Thermal Decomposition, Amalgamation and AAS |
| Chemistry: Inorganic Sample Analysis | 537 | Analysis of Perchlorate by LC-MS-MS |
| Chemistry: Inorganic Sample Analysis | 545 | Specific Conductance |
| Chemistry: Inorganic Sample Analysis | 553 | Determination of TOC in Water by EPA Method 415.3 |
| Chemistry: Inorganic Sample Analysis | 560 | Total Alkalinity by Titrimetric Method |
| Chemistry: Inorganic Sample Analysis | 565 | Sulfide Analysis by Titration |
| Chemistry: Inorganic Sample Analysis | 570 | Hardness, Total (mg/l as CaCO3) |
| Chemistry: Inorganic Sample Analysis | 575 | Determination of Ferrous Iron by Titration with Dichromate |
| Chemistry: Inorganic Sample Analysis | 580 | pH Determination |
| Chemistry: Inorganic Sample Analysis | 582 | pH in Solid Samples |
| Chemistry: Inorganic Sample Analysis | 590 | Ammonia Analysis |
| Chemistry: Inorganic Sample Analysis | 592 | Total Kjeldahl Nitrogen in Water |
| Chemistry: Inorganic Sample Analysis | 594 | Analysis of Nitrite and Nitrate in Water by Cadmium Reduction |
| Chemistry: Inorganic Sample Analysis | 596 | Analysis of Total Phosphorus in Water |
| Safety, Health & Environmental Compliance: Standard Practices | 704 | Inspection Procedures for Emergency Eyewash and Shower Units |
| Safety, Health & Environmental Compliance: Standard Practices | 706 | Laboratory Waste Management |
| Safety, Health & Environmental Compliance: Standard Practices | 712 | Inspection Procedures for the Satellite and 90-Day Hazardous Waste Accumulation Areas |
| Safety, Health & Environmental Compliance: Standard Practices | 716 | Cleaning and Recycling of Empty Containers |
| Safety, Health & Environmental Compliance: Standard Practices | 730 | Neutralization of Aqueous Wastes |
| Safety, Health & Environmental Compliance: Standard Practices | 745 | Maintenance and Inspection of Spill Cleanup Equipment |
| Safety, Health & Environmental Compliance: Standard Practices | 747 | Laboratory Chemical Inventory Maintenance |
| Safety, Health & Environmental Compliance: Standard Practices | 748 | Chemical Adoption Program |
| Safety, Health & Environmental Compliance: Standard Practices | 750 | Inspection Procedures for Fire Extinguishers |
| Safety, Health & Environmental Compliance: Standard Practices | 760 | Maintenance and Upkeep of Radioactive Sources |
| Safety, Health & Environmental Compliance: Standard Practices | 765 | Procedure for Spill Response and Exposure Control for Methylene Chloride |
| Safety, Health & Environmental Compliance: Standard Practices | 770 | Handling Cryogenic Materials |
| Safety, Health & Environmental Compliance: Standard Practices | 775 | Handling and Disposal of Broken Glassware |
| Safety, Health & Environmental Compliance: Standard Practices | 780 | Procedure for Chemical Spill Response and Cleanup |
| Safety, Health & Environmental Compliance: Standard Practices | 785 | Procedure for Storage, Monitoring and Handling of Peroxide Forming Chemicals |
| Quality Assurance | 805 | Refrigerator temperature monitoring |
| Quality Assurance | 810 | Internal Audits of Laboratories |
| Quality Assurance | 817 | Accuracy Check of Laboratory Thermometers |
| Quality Assurance | 820 | Lab Discrepancy/Corrective Action Report Procedure |
| Quality Assurance | 825 | Deionized water monitoring |
| Quality Assurance | 830 | Notification of exceeding safe drinking water mcl |
| Quality Assurance | 835 | Chromatographic integration procedures |
| Quality Assurance | 840 | Notebook documentation and control |
| Quality Assurance | 845 | Final Chemistry Data Review & Report Preparation |
| Quality Assurance | 850 | Preparation and usage of SOPs |
| Quality Assurance | 880 | Initial & continuing demonstrations of proficiency (Chemistry) |
| Quality Assurance | 885 | Initial & continuing demonstrations of proficiency (Biology) |
| Quality Assurance | 890 | Calibration & maintenance of automatic pipets |
| Quality Assurance | 892 | Initial Instrument Calibration Guidelines |
| Biology: Marine Technical | 1001 | Sea urchin & sand dollar fertilization toxicity test |
| Biology: Marine Technical | 1004 | Red abalone larval development toxicity test |
| Biology: Freshwater Technical - Chronic | 1020 | Fathead minnow, larval survival & growth test |
| Biology: Freshwater Technical - Chronic | 1022 | Green alga, growth test |
| Biology: Freshwater Technical - Acute | 1030 | Fathead minnow acute survival test |
| Biology: Freshwater Technical - Acute | 1032 | Daphnia magna acute survival test |
| Biology: General Activity | 1060 | Calibration and Operation of pH meter |
| Biology: General Activity | 1061 | Calibration and Operation of Dissolved Oxygen Meters |
| Biology: General Activity | 1063 | Calibration and Operation of Conductivity Meters |
| Biology: General Activity | 1067 | Calibration and Operation of Refractometers |
| Biology: General Activity | 1073 | Calibration & Operation of Oakton Portable pH/CON 10 Series Meter |
| Biology: General Activity | 1080 | Preparation of standard dilution water (freshwater) |
| Biology: General Activity | 1081 | Preparation of foods (freshwater) |
| Biology: General Activity | 1082 | Culturing of selenastrum capricornatum |
| Biology: General Activity | 1085 | Glass & Plasticware Cleaning for Marine Toxicity Testing |
| Biology: General Activity | 1086 | Kelp collection, preparation & feeding |
| Biology: Microbiology | 1101 | Membrane filtration coliform analysis |
| Biology: Microbiology | 1102 | Multiple-tube fermentation analysis for total and fecal coliform |
| Biology: Microbiology | 1103 | Colilert coliform and E. coli water analysis |
| Biology: Microbiology | 1106 | Volunteer monitoring of surface waters for bacteria |
| Biology: Microbiology | 1107 | Enterolert entercocci water analysis |
| Biology: Microbiology | 1108 | Heterotrophic plate count for bacteria in water |
| Biology: Microbiology | 1109 | Microbiology Autoclaves |
| Biology: Miscellaneous Biology | 1131 | Analysis of Ash Free Dry Mass |
| Biology: Miscellaneous Biology | 1133 | Biochemical oxygen demand-BOD |
| Biology: Miscellaneous Biology | 1134 | Chlorophyll |
| Biology: Miscellaneous Biology | 1135 | Total & free residual chlorine - Hach DPD |
| Field Sampling: Water | 1220 | Groundwater Monitoring Well Sampling |
| Field Sampling: Water | 1222 | Routine Sampling and Analysis of Drinking water |
| Miscellarous Immunoassay | 1305 | Microcystin by ELISA Analysis |