EPA has finalized the Biological Evaluation for Diazinon. The final evaluation is based on interim methods developed in conjunction with Fish and Wildlife Service and National Marine Fisheries Service in response to the National Academy of Science report on assessing risks to threatened and endangered species from pesticides. 

Response to Comments on the Biological Evaluations for Chlorpyrifos, Diazinon and Malathion (PDF) (20 pp, 977 K, About PDF)

• Diazinon Executive Summary for ESA Assessment
• Chapter 1: Diazinon Problem Formulation for ESA Assessment
  • Attachments
  • Appendices
• Chapter 2: Diazinon Effects Characterization for ESA Assessment
  • Attachments
  • Appendices
• Chapter 3: Diazinon Exposure Characterization for ESA Assessment
  • Attachments
  • Appendices
• Chapter 4: Diazinon Effects Determinations for ESA Assessment
  • Attachments
  • Appendices

Diazinon Executive Summary for ESA Assessment (DOCX)(4 pp, 31 K)

Chapter 1: Diazinon Problem Formulation for ESA Assessment (DOCX)(64 pp, 859 K)

Attachments

• ATTACHMENT 1-1: Ecological Incidents (DOCX)(2 pp, 17 K)
• ATTACHMENT 1-2: CDL Crosswalk (DOCX)(5 pp, 27 K)
• ATTACHMENT 1-3: Method for Establishing the Use Footprint (DOCX)(10 pp, 31 K)
• ATTACHMENT 1-4: Process for Determining Effects Thresholds (DOCX)(5 pp, 27 K)
• ATTACHMENT 1-5: Method for Deriving Species Sensitivity Distributions for Use in Pesticide Effects Determinations for Listed Species (DOCX)(22 pp, 228 K)
• ATTACHMENT 1-6: Co-Occurrence Analysis (XLSX)(1 pp, 1.46 MB)
• ATTACHMENT 1-7: Methodology for Estimating Exposures to Terrestrial Animals (DOCX)(18pp, 86 K)
• ATTACHMENT 1-8: Review of Open Literature Toxicity Studies for Pilot Chemical Biological Evaluations (DOCX)(4 pp, 132 K)
• ATTACHMENT 1-9: Applying a Weight-of-Evidence Approach to Support Step 2 Effects Determinations (DOCX)(17 pp, 4.20 MB)
• ATTACHMENT 1-10: Aquatic Bin Assignments (XLSX)(1 pp, 343 K)
• ATTACHMENT 1-11: Biological Information on Listed Species of Fish and Model Parameterization for Pesticide Effects Determinations (DOCX)(43 pp, 92 K)
  • ATTACHMENT 1-11 Supplemental Information 2: Fish Attribute Template (XLSX)(1 pp, 904 K)
  • ATTACHMENT 1-11 Supplemental Information 3: Federally Listed Fish Attribute Database (XLSX)(1 pp, 857 K)
• ATTACHMENT 1-12: Biological Information on Listed Species of Aquatic Invertebrates and Model Parameterization for Pesticide Effects Determinations (DOCX)(41 pp, 105)
  • ATTACHMENT 1-12 Supplemental Information 1: Federally Listed Aquatic Invertebrate Database (XLSX)(1 pp, 904 K)
  • ATTACHMENT 1-12 Supplemental Information 2: Aquatic Invertebrate Attribute Template (DOCX)(41 pp, 106 K)
• ATTACHMENT 1-13: Biological Information on Listed Species of Marine Mammals and Model Parameterization for Pesticide Effects Determinations (DOCX)(67 pp, 115 K)
• ATTACHMENT 1-14: Biological Information on Listed Species of Marine Reptiles and Model Parameterization for Pesticide Effects Determinations (DOCX)(30 pp, 49 K)
• ATTACHMENT 1-15: Biological Information on Listed Species of Corals and Model Parameterization for Pesticide Effects Determinations (DOCX)(9 pp, 37 K)
• ATTACHMENT 1-16: Biological Information on Listed Species of Birds and Model Parameterization for Pesticide Effects Determinations (DOCX)(318 pp, 597 K)
• ATTACHMENT 1-17: Biological Information on Listed Species of Mammals (excluding marine species) and Model Parameterization for Pesticide Effects Determinations (DOCX)(250 pp, 363 K)
• ATTACHMENT 1-18: Biological Information on Listed Species of Reptiles (excluding marine species) and Model Parameterization for Pesticide Effects Determinations (DOCX)(104 pp, 174 K)
• ATTACHMENT 1-19: Biological Information on Listed Species of Amphibians and Model Parameterization for Pesticide Effects Determinations (DOCX)(123 pp, 209 K)
• ATTACHMENT 1-20: Biological Information on Listed Species of Terrestrial Invertebrates and Model Parameterization for Pesticide Effects Determinations (DOCX)(50 pp, 172 K)
  • ​ATTACHMENT 1-20 Supplemental Information 3: Federally Terrestrial Invertebrate Database (XLSX)(1 pp, 454 K)
• ATTACHMENT 1-21: Biological Information on Listed Species of Plants and Lichens and Model Parameterization for Pesticide Effects Determinations (DOCX)(6 pp, 27 K)
  • ATTACHMENT 1-21 Supplemental Information 1: Federally Listed Plant Attribute Database (XLSX)(1 pp, 113 MB)
  • ATTACHMENT 1-21 Supplemental Information 2: Summary Plant and Lichen Information (XLSX)( 1 pp, 531 K)
• ATTACHMENT 1-22: Effects Arrays (DOCX)(1 pp, 17 K)

Appendices

• APPENDIX 1-1: Regulatory History and Past Assessments for Diazinon (DOCX)(6 pp, 34 K)
• APPENDIX 1-2: List of Current Diazinon Registrations (Registration Numbers and Label Stamp Dates) (DOCX)(1 pp, 30 K)
• APPENDIX 1-3: Master Use Summary Table for Diazinon (DOCX)(6 pp, 59 K)
• APPENDIX 1-4: Tank Mixes Specified on Diazinon Product Labels (DOCX)(6 pp, 41 K)
• APPENDIX 1-5: Commitment Letters from Diazinon Registrants (PDF)(1 pp, 125 K, About PDF)
• APPENDIX 1-6: Use Site, General Land Cover Class, and HUC2 Matrix for Diazinon (DOCX)(5 pp, 26 K)
  • APPENDIX 1-6a: General Land Cover Class and Use Site for Diazinon (XLSX)(1 pp, 23 K)
• APPENDIX 1-7: Diazinon Scenario Development (DOCX)(6 pp, 38 K)
• APPENDIX 1-8: Usage Data for Diazinon (DOCX)(2 pp, 26 K)
• APPENDIX 1-9: Degradate Line of Evidence (DOCX)(10 pp, 48 K)
• APPENDIX 1-10: Summary of Diazinon Monitoring Data (DOCX)(27 pp, 222 K)
• APPENDIX 1-11: Multi-A.I. Formulation Analysis for Diazinon (DOCX)(1 pp, 12 K)
• APPENDIX 1-12: ECOTOX Mixture Studies (Diazinon) (DOCX)(2 pp,18 K)

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Chapter 2: Diazinon Effects Characterization for ESA Assessment (DOCX)(151 pp, 2 MB)

Attachments:

• ATACHMENT 1-1: Ecological Incidents (DOCX) (2 pp, 17 K)
• ATTACHMENT 1-4: Process for Determining Effects Thresholds (DOCX)(5 pp, 27 K)
• ATTACHMENT 1-5: Method for Deriving Species Sensitivity Distributions for Use in Pesticide Effects Determinations for Listed Species (DOCX)(22 pp, 228 K)

Appendices

• APPENDIX 2-1: Data Used in the Data Array (XLSX)(1 pp, 826 K)
• APPENDIX 2-2: Accepted ECOTOX Database (XLSX)(1 pp, 1.39 MB)
• APPENDIX 2-3 Open Literature Review for Diazinon (DOCX)(52 pp, 958 K)
• APPENDIX 2-4: OPPIN Bibliography for Diazinon (PDF)(222 pp, 445 K)
• APPENDIX 2-5: Rejected ECOTOX Bibliography (DOCX)(114 pp, 1.3 MB)
• APPENDIX 2-6: Diazinon Species Sensitivity Distribution Analysis for Fish (DOCX)(xx pp, yy K)
• APPENDIX 2-7: Additional Effects Arrays for Diazinon (DOCX)(14 pp, 14 K)
• APPENDIX 2-8: Diazinon Species Sensitivity Distribution Analysis for Aquatic Invertebrates (DOCX)(13 pp, 57 K)
• APPENDIX 2-9: Diazinon Species Sensitivity Distribution Analysis for Birds (DOCX)(5 pp, 27 K)

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Chapter 3: Diazinon Exposure Characterization for ESA Assessment (DOCX)(65 pp, 2 MB, About PDF)

Attachments

• ATTACHMENT 3-1: Background Document: Aquatic Exposure Estimation for Endangered Species (DOCX)(52 pp, 1.81 MB)
  • ATTACHMENT 3-1 Supplemental Information 1: Static Bin Analysis (XLSX)(1 pp, 85 K)

Appendices

• APPENDIX 1-3: Master Use Summary Table for Diazinon (DOCX) (6 pp, 59 K)
• APPENDIX 1-6: Use Site, General Land Cover Class, and HUC2 Matrix for Diazinon (DOCX) (5 pp, 26 K)
• APPENDIX 1-7: Diazinon Scenario Development (DOCX) (6 pp, 38 K)
• APPENDIX 3-1: Environmental Transport and Fate Data Analysis for Diazinon (DOCX) (24 pp, 165 K)
• APPENDIX 3-2: Diazinon Fate Open Literature Review (XLSX)(1 pp, 5.4 MB)
• APPENDIX 3-3: Spray Drift Considerations for Diazinon (DOCX)(2 pp, 20 K)
• APPENDIX 3-4a: Diazinon Aquatic Modeling Inputs (XLSX)(1 pp, 11 MB)
• APPENDIX 3-4b: Aquatic EECs Ste 2 (XLSX) (1 pp, 226 K)
• APPENDIX 3-4c: Aquatic EECs PFAM (XLSX) (1 pp, 4 MB)
• APPENDIX 3-4d: Aquatic EECs Sensitivity Analysis (XLSX) (1 pp, 6 MB)
• APPENDIX 3-4e: Example Aquatic Modeling Figure with Effects Endpoints (XLSX)(1 pp, 1 G)
• APPENDIX 3-4f: PWC Postprocessor Output (ZIP)(1 pp, 6 MB)
• APPENDIX 3-5: Diazinon Downstream Dilution (DOCX)(71 pp, 308 K)
• APPENDIX 3-6: Input Parameters for Weight of Evidence Matrices (XLSX) (1 pp, 106 K)

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Chapter 4: Diazinon Effects Determinations for ESA Assessment (DOCX)(45 pp, 144 K, About PDF)

Attachments

• ATTACHMENT 4-1: Terrestrial and Aquatic Weight of Evidence Matrices: Tool Methodology and Description (DOCX)(31 pp, 2.09 MB)
• ATTACHMENT 4-2: Regression Analysis of Exposure Response Data from Chronic Aquatic Toxicity Tests for Chlorpyrifos, Diazinon and Malathion (DOCX)(10 pp, 86 K)
  • ATTACHMENT 4-2 Supplemental Information 1: Appendix A- Response Curve Modeling (DOCX)(11 pp, 296 K)
  • ATTACHMENT 4-2 Supplemental Information 2: Appendix B- Guidelines for Assessing Relative Quality of TRAP Analysis Graphs (DOCX)(8 pp, 281 K)
  • ATTACHMENT 4-2 Supplemental Information 3: Appendix C- Index and Tabular Results (XLSX)(1 pp, 28 K)
  • ATTACHMENT 4-2 Supplemental Information 4: Appendix D- TRAP Analysis Graphs and ECx Values (PDF)(60 pp, 799 K, About PDF)
• ATTACHMENT 4-3: Application of Web-ICE to Assess Risks of National Pesticide Registrations to Federally Listed Species (DOCX)(9 pp, 44 K)
  • ATTACHMENT 4-3 Supplemental Information 1: Acute Toxicity Prediction to Threatened and Endangered Species Using Interspecies Correlation Estimation (ICE) Models (DOCX)(26 pp, 102 K)
    • ATTACHMENT 4-3 Supplemental Information 1.1 (DOCX)(5 pp, 466 K)
    • ATTACHMENT 4-3 Supplemental Information 1.2 (XLSX)(1 pp, 155 K)
  • ATTACHMENT 4-3 Supplemental Information 2: Analysis of Predicted vs. Empirical SSDs for Malathion (DOCX)(21 pp, 87 K)
  • ATTACHMENT 4-3 Supplemental Information 3: Analysis of Predicted vs. Empirical SSDs for Chlorpyrifos (DOCX)(19pp, 81 K)
  • ATTACHMENT 4-3 Supplemental Information 4: Analysis of Predicted vs. Empirical SSDs for Diazinon (DOCX)(19 pp, 84 K)

Appendices

• APPENDIX 4-1: Effects Determination Tables (XLSX)(1 pp, 407y K)
• APPENDIX 4-2: Mixtures Analysis for Diazinon (DOCX)(12 pp, 154 K)
• APPENDIX 4-3: Weight of Evidence Matrices
  • APPENDIX 3-6: Input Parameters for Weight of Evidence Matrices (XLSX)(1 pp, 106 K)
  • APPENDIX 4-3a: Amphibians_All_DIA (XLSX)(1 pp, 1.7 MB)
  • APPENDIX 4-3b: Reptiles_DIA (XLSX)(1 pp, 1.6 MB)
  • APPENDIX 4-3c: Birds_Passerine_DIA (XLSX)(1 pp, 1.7 MB)
  • APPENDIX 4-3d: Birds_All other orders_DIA (XLSX)(1 pp, 1.26 MB)
  • APPENDIX 4-3e: Mammals_All_DIA (XLSX)(1 pp, 1.12 MB)
  • APPENDIX 4-3f: Terrestrial Invertebrates_Arachnids and Insects_DIA (XLSX)(1pp, 1.14 MB)
  • APPENDIX 4-3g: Terrestrial Invertebrates_Snails_DIA (XLSX)(1 pp, 1.52 MB)
  • APPENDIX 4-3h: Fish_Cypriniformes_DIA (XLSX)(1 pp, 645 K)
  • APPENDIX 4-3i: Fish_Salmoniformes_DIA (XLSX)(1 pp, 594 K)
  • APPENDIX 4-3j: Fish_All other orders_DIA (XLSX)(1 pp, 656 K)
  • APPENDIX 4-3k: Aquatic Invertebrates_Insects and Crustaceans_DIA (XLSX)(1x pp, 652 K)
  • APPENDIX 4-3l: Aquatic Invertebrates_Mollusks_Unionida1_DIA (XLSX)(1 pp, 615 K)
  • APPENDIX 4-3m: Aquatic Invertebrates_Mollusks_Unionida2_DIA (XLSX)(1 pp, 629 K)
  • APPENDIX 4-3n: Aquatic Invertebrates_All other orders_DIA (XLSX)(1 pp, 611 K)
  • APPENDIX 4-3o: MARINE species_Coral_DIA (XLSX)(1 pp, 577 K)
  • APPENDIX 4-3p: MARINE species_Fish and Other Invertebrates_DIA (XLSX)(1 pp, 589 K)
  • APPENDIX 4-3q: Plants_General _DIA (XLSX)(1 pp, 514 K)
  • APPENDIX 4-3r: Plants_Herbaceous Dicot_1_DIA (XLSX)(1 pp, 744 K)
  • APPENDIX 4-3s: Plants_Herbaceous Dicot_2_DIA (XLSX)(1 p, 731 K)
  • APPENDIX 4-3t: Plants_Herbaceous Dicot_3_DIA (XLSX)(1 pp, 669 K)
  • APPENDIX 4-3u: Plants_Herbaceous Dicot_4_DIA (XLSX)(1 pp, 649 K)
  • APPENDIX 4-3v: Plants_Herbaceous Dicot_5_DIA (XLSX)(1 pp, 493 K)
  • APPENDIX 4-3w: Plants_Herbaceous Monocot _DIA (XLSX)(1 pp, 686 K)
  • APPENDIX 4-3x: Plants_Woody Dicot_1_DIA (XLSX)(1 pp, 733 K)
  • APPENDIX 4-3y: Plants_Woody Dicot_2_DIA (XLSX)(1 pp, 595 K)
  • APPENDIX 4-3z: Plants_Woody Dicot_3_DIA (XLSX)(1 pp, 614 K)
  • APPENDIX 4-3aa: Plants_Woody Dicot_4_DIA (XLSX)(1 pp, 580 K)
  • APPENDIX 4-3bb: Plants_Woody Monocot_DIA (XLSX)(1 pp, 479)
• APPENDIX 4-4: Risk Assessment for Cattle Ear Tag Use (DOCX)(68 pp, 282 K)
• APPENDIX 4-5: Analysis of Mosquito Adulticide Use (DOCX) (1 pp, 11 K)
• APPENDIX 4-6: Analysis of Seed Treatment and Granular Uses (DOCX)(1 pp, 11 K)
• APPENDIX 4-7: Refined Risk Analyses for 11 Listed Birds Exposed to Diazinon (DOCX)(23 pp, 127 K)
  • APPENDIX 4-7 Supplemental Information 1: TIM/MCnest Metadata (DOCX)(48 pp, 118 K)
  • APPENDIX 4-7 Supplemental Information 2: GAP Land covers assigned as preferred habitats (XLSX)(1 pp, 28 K)

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