EPA has finalized the Biological Evaluation for chlorpyrifos. The final evaluation is based on interim methods developed in conjunction with Fish and Wildlife Service and National Marine Fisheries Service in response to the National Academy of Science report on assessing risks to threatened and endangered species from pesticides.

Response to Comments on the Biological Evaluations for Chlorpyrifos, Diazinon and Malathion (PDF) (20 pp, 978 K,  About PDF)

• Chlorpyrifos Executive Summary for ESA Assessment
• Chlorpyrifos Problem Formulation for ESA Assessment
  • Attachments
  • Appendices
• Chlorpyrifos Effects Characterization for ESA Assessment
  • Attachments
  • Appendices
• Chlorpyrifos Exposure Characterization for ESA Assessment
  • Attachments
  • Appendices
• Chlorpyrifos Effects Determinations for ESA Assessment
  • Attachments
  • Appendices

Chlorpyrifos Executive Summary for ESA Assessment (DOCX)(4 pp, 32 K)

Chapter 1:  Chlorpyrifos Problem Formulation for ESA Assessment (DOCX)(58 pp, 1.21MB)

Attachments

• ATTACHMENT 1-1: Ecological Incidents (DOCX)(2 pp, 17 K)
• ATTACHMENT 1-2: CDL Crosswalk (DOCX)(5 pp, 27 K)
• ATTACHMENT 1-3: Method for Establishing the Use Footprint (DOCX)(40 pp, 102 K)
• ATTACHMENT 1-4: Process for Determining Effects Thresholds (DOCX)(5 pp, 27 K)
• ATTACHMENT 1-5: Method for Deriving Species Sensitivity Distributions for Use in Pesticide Effects Determinations for Listed Species (DOCX)(22 pp, 228 K)
• ATTACHMENT 1-6: Co-Occurrence Analysis (XLSX) (1 pg, 1.46 MB)
• ATTACHMENT 1-7: Methodology for Estimating Exposures to Terrestrial Animals (DOC) (18 pp, 86 K)
• ATTACHMENT 1-8: Review of Open Literature Toxicity Studies for Pilot Chemical Biological Evaluations (DOCX)(4 pp, 132 K)
• ATTACHMENT 1-9: Applying a Weight-of-Evidence Approach to Support Step 2 Effects Determinations (DOCX) (17 pp, 4.20 MB)
• ATTACHMENT 1-10: Aquatic Bin Assignments (XLSX) (1 pg, 343 K)
• ATTACHMENT 1-11: Biological Information on Listed Species of Fish and Model Parameterization for Pesticide Effects Determinations (DOCX) (43 pp, 92 K)
  • ATTACHMENT 1-11 Supplemental Information 2: Fish Attribute Template (XLSX)(1 pg, 21 K)
  • ATTACHMENT 1-11 Supplemental Information 3: Federally Listed Fish Attribute Database (XLSX)(1 pg, 857 K)
• ATTACHMENT 1-12: Biological Information on Listed Species of Aquatic Invertebrates and Model Parameterization for Pesticide Effects Determinations (DOCX) (41 pp, 105 K)
  • ATTACHMENT 1-12 Supplemental Information 1: Federally Listed Aquatic Invertebrate Database (XLSX) (1 pg, 903 K)
  • ATTACHMENT 1-12 Supplemental Information 2: Aquatic Invertebrate Attribute Template (XLSX)( 1 pg,  20 K)
• ATTACHMENT 1-13: Biological Information on Listed Species of Marine Mammals and Model Parameterization for Pesticide Effects Determinations (DOCX)(67 pp, 115 K)
• ATTACHMENT 1-14: Biological Information on Listed Species of Marine Reptiles and Model Parameterization for Pesticide Effects Determinations (DOCX)(30 pp, 49 K)
• ATTACHMENT 1-15: Biological Information on Listed Species of Corals and Model Parameterization for Pesticide Effects Determinations (DOCX)(9 pp, 37 K)
• ATTACHMENT 1-16: Biological Information on Listed Species of Birds and Model Parameterization for Pesticide Effects Determinations (DOCX)(318 pp, 596 K)
• ATTACHMENT 1-17: Biological Information on Listed Species of Mammals (excluding marine species) and Model Parameterization for Pesticide Effects Determinations (DOCX)(250 pp, 363 K)
• ATTACHMENT 1-18: Biological Information on Listed Species of Reptiles (excluding marine species) and Model Parameterization for Pesticide Effects Determinations (DOCX)(104 pp, 174 K)
• ATTACHMENT 1-19: Biological Information on Listed Species of Amphibians and Model Parameterization for Pesticide Effects Determinations (DOCX)(123 pp, 209 K)
• ATTACHMENT 1-20: Biological Information on Listed Species of Terrestrial Invertebrates and Model Parameterization for Pesticide Effects Determinations (DOCX) (50 pp, 172 K)
• ATTACHMENT 1-21: Biological Information on Listed Species of Plants and Lichens and Model Parameterization for Pesticide Effects Determinations (DOCX) (6 pp, 27 K)
  • ATTACHMENT 1-21 Supplemental Information 1: Federally Listed Plant Attribute Database (XLSX) (4 pp, 113 MB)
  • ATTACHMENT 1-21 Supplemental Information 2: Summary Plant and Lichen Information (XLSX) (1 pg, 531 K)
• ATTACHMENT 1-22: Effects Arrays (DOCX)(1 pg,  17K)

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Appendices

• APPENDIX 1-1: Regulatory History and Past Assessments for Chlorpyrifos (DOCX)(3 pp, 24 K)
• APPENDIX 1-2: List of Current Chlorpyrifos Registrations (Registration Numbers and Label Stamp Dates) (DOCX)(4 pp, 29 K)
• APPENDIX 1-3: Master Use Summary Table for Chlorpyrifos (DOCX)(47 pp, 134 K)
• APPENDIX 1-4: Tank Mixes Specified on Chlorpyrifos Product Labels (DOCX)(74 pp, 74 K)
• APPENDIX 1-5: Commitment Letters from Chlorpyrifos Registrants (PDF)(123 pp, 3.43 MB, About PDF)
• APPENDIX 1-6: Use Site, General Land Cover Class, and HUC2 Matrix for Chlorpyrifos (DOCX) (17 pp, 35 K)
• APPENDIX 1-7: Chlorpyrifos Scenario Development (DOCX)(40 pp, 114 K)
• APPENDIX 1-8: Usage Data for Chlorpyrifos (DOCX)(12 pp, 2.02 MB)
• APPENDIX 1-9: Degradate Line of Evidence (DOCX)(9 pp, 36 K)
• APPENDIX 1-10: Summary of Chlorpyrifos Monitoring Data (DOCX)(18 pp, 73 K)
• APPENDIX 1-11: Multi-A.I. Formulation Analysis for Chlorpyrifos (DOCX)(4 pp, 27 K)
• APPENDIX 1-12: ECOTOX Mixture Studies (Chlorpyrifos) (DOCX)(7 pp, 26 K)

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Chapter 2: Chlorpyrifos Effects Characterization for ESA Assessment (DOCX)(268 pp, 6.74 MB)

Attachments

• ATTACHMENT 1-1: Ecological Incidents (DOCX)(2 pp, 17 K)
• ATTACHMENT 1-4: Process for Determining Effects Thresholds (DOCX)(5 pp, 27 K)
• ATTACHMENT 1-5: Method for Deriving Species Sensitivity Distributions for Use in Pesticide Effects Determinations for Listed Species (DOCX)(22 pp, 228 K)
• ATTACHMENT 1-8: Review of Open Literature Toxicity Studies for Pilot Chemical Biological Evaluations (DOCX)(4 pp, 132 K)
• ATTACHMENT 1-22: Effects Arrays (DOCX)(1 pg, 17 K)

Appendices

• APPENDIX 2-1: Data Used in the Data Array (XLSX)(1 pg, 3.74 MB)
• APPENDIX 2-2: Accepted ECOTOX Database (XLSX)(2 pp, 4.46 MB)
• APPENDIX 2-3 Open Literature Review for Chlorpyrifos (DOCX)(113 pp, 468 K)
• APPENDIX 2-4: OPPIN Bibliography for Chlorpyrifos (DOCX)(433 pp, 381 K)
• APPENDIX 2-5: Rejected ECOTOX Bibliography (DOCX)(2,015 pp, 10.4 MB)
• APPENDIX 2-6: Chlorpyrifos Species Sensitivity Distribution Analysis for Fish (DOCX)(22 pp, 192 K)
• APPENDIX 2-7: Additional Effects Arrays for Chlorpyrifos (DOCX)(10 pp, 2.17 MB)
• APPENDIX 2-8: Chlorpyrifos Species Sensitivity Distribution Analysis for Aquatic Invertebrates (DOCX)(24 pp, 562 K)
• APPENDIX 2-9: Chlorpyrifos Species Sensitivity Distribution Analysis for Birds (DOCX)(3 pp, 36 K)

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Chapter 3:  Chlorpyrifos Exposure Characterization for ESA Assessment (DOCX) (53 pp, 1.45 MB)

Attachments

• ATTACHMENT 3-1: Background Document: Aquatic Exposure Estimation for Endangered Species (DOCX) ( 52 pp,  181 MB)
  • ATTACHMENT 3-1 Supplemental Information 1: Static Bin Analysis (XLSX)(1 pg, 85 K)

Appendices

• APPENDIX 1-3: Master Use Summary Table for Chlorpyrifos (DOCX)(47 pp, 133 K)
• APPENDIX 1-6: Use Site, General Land Cover Class, and HUC2 Matrix for Chlorpyrifos (DOCX) (18 pp, 35 K)
• APPENDIX 1-7: Chlorpyrifos Scenario Development (DOCX)(40 pp, 114 K)
• APPENDIX 3-1: Environmental Transport and Fate Data Analysis for Chlorpyrifos (DOCX)(52 pp, 1.81 MB)
• APPENDIX 3-2: Chlorpyrifos Fate Open Literature Review (XLSX)(1 pg, 126 K)
• APPENDIX 3-3: Spray Drift Considerations for Chlorpyrifos (DOCX) (9 pp, 128 K)
• APPENDIX 3-4: Aquatic EECs (XLSX) ( 1 pg,  3.86 MB)
  • APPENDIX 3-4f: PWC Postprocessor Output (ZIP)(Please save this file prior to opening)(1 file, 3.43 GB)
• APPENDIX 3-5: Chlorpyrifos Downstream Dilution (DOCX) (1 pg, 15 K)
• APPENDIX 3-6: Input Parameters for Weight of Evidence Matrices (XLSX) (1 pg, 109 K)

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Chapter 4: Chlorpyrifos Effects Determinations for ESA Assessment (DOCX)(45 pp, 151 K)

Attachments

• ATTACHMENT 4-1: Terrestrial and Aquatic Weight of Evidence Matrices: Tool Methodology and Description (DOCX)(31 pp, 2.09 MB)
• ATTACHMENT 4-2: Regression Analysis of Exposure Response Data from Chronic Aquatic Toxicity Tests for Chlorpyrifos, Diazinon and Malathion (DOCX)(10 pp, 86 K)
  • ATTACHMENT 4-2 Supplemental Information 1: Appendix A- Response Curve Modeling (DOCX)(11 pp, 296 K)
  • ATTACHMENT 4-2 Supplemental Information 2: Appendix B- Guidelines for Assessing Relative Quality of TRAP Analysis Graphs (DOCX)(8 pp, 281 K)
  • ATTACHMENT 4-2 Supplemental Information 3: Appendix C- Index and Tabular Results (XLSX)(1 pg, 28 K)
  • ATTACHMENT 4-2 Supplemental Information 4: Appendix D- TRAP Analysis Graphs and ECx Values (PDF)(60 pp, 799K)
• ATTACHMENT 4-3: Application of Web-ICE to Assess Risks of National Pesticide Registrations to Federally Listed Species (DOCX)(9 pp, 44 K)
  • ATTACHMENT 4-3 Supplemental Information 1: Acute Toxicity Prediction to Threatened and Endangered Species Using Interspecies Correlation Estimation (ICE) Models (DOCX)(5 pp, 466 K)
  • ATTACHMENT 4-3 Supplemental Information 1.1 (DOCX)(26 pp, 101 K)
  • ATTACHMENT 4-3 Supplemental Information 1.2 (XLSX)(1 pg, 155 K)
• ATTACHMENT 4-3 Supplemental Information 2: Analysis of Predicted vs. Empirical SSDs for Malathion (DOCX)(21 pp, 85K)
• ATTACHMENT 4-3 Supplemental Information 3: Analysis of Predicted vs. Empirical SSDs for Chlorpyrifos (DOCX)(19 pp, 81 K)
• ATTACHMENT 4-3 Supplemental Information 4: Analysis of Predicted vs. Empirical SSDs for Diazinon (DOCX)(19 pp, 85 K)

Appendices

• APPENDIX 4-1: Effects Determination Tables (XLSX)( 1 pg,  459 K)
• APPENDIX 4-2: Mixtures Analysis for Chlorpyrifos (DOCX)(12 pp, 611 K)
• APPENDIX 4-3: Weight of Evidence Matrices 
  • APPENDIX 3-6: Input Parameters for Weight of Evidence Matrices (XLSX)(1 pg, 109 K)
  • APPENDIX 4-3a: Amphibians_All_CPY (XLSX)(1 pg, 1.72 MB)
  • APPENDIX 4-3b: Reptiles_CPY (XLSX)(1 p, 1.54 MB)
  • APPENDIX 4-3c: Birds_Passerine_CPY (XLSX)(1 pg, 1.48 MB)
  • APPENDIX 4-3d: Birds_All other orders_CPY (XLSX)(1 pg, 1.27 MB)
  • APPENDIX 4-3e: Mammals_All_CPY (XLSX)(1 pg, 1.09 MB)
  • APPENDIX 4-3f: Terrestrial Invertebrates_Arachnids and Insects_CPY (XLSX)(1 pg, 1.18 MB)
  • APPENDIX 4-3g: Terrestrial Invertebrates_Snails_CPY (XLSX)(1 pg, 1.38 MB)
  • APPENDIX 4-3h: Fish_Cypriniformes_CPY (XLSX)(1 pg, 624 K)
  • APPENDIX 4-3i: Fish_Salmoniformes_CPY (XLSX)(1 pg, 596 K)
  • APPENDIX 4-3j: Fish_All other orders_CPY (XLSX)(1 pg, 691 K)
  • APPENDIX 4-3k: Aquatic Invertebrates_Insects and Crustaceans_CPY (XLSX)(1 pg, 597 K)
  • APPENDIX 4-3l: Aquatic Invertebrates_Mollusks_Unionida1_CPY (XLSX)(1 pg, 612 K)
  • APPENDIX 4-3m: Aquatic Invertebrates_Mollusks_Unionida2_CPY (XLSX)(1 pg, 666 K)
  • APPENDIX 4-3n: Aquatic Invertebrates_All other orders_CPY (XLSX)(1 pg, 998 K)
  • APPENDIX 4-3o: MARINE species_Coral_CPY (XLSX)(1 pg, 575 K)
  • APPENDIX 4-3p: MARINE species_Fish and Other Invertebrates_CPY (XLSX)(1 pg, 578 K)
  • APPENDIX 4-3q: Plants_General_Ad-Tr_CPY (XLSX)(1 pg, 539 K)
  • APPENDIX 4-3r: Plants_Herbaceous Dicot_Ab-As_CPY (XLSX)(1 pg, 530 K)
  • APPENDIX 4-3s: Plants_Herbaceous Dicot_As-Ci_CPY (XLSX)(1 pg, 527 K)
  • APPENDIX 4-3t: Plants_Herbaceous Dicot_Ci-Er_CPY (XLSX)(1 pg, 535 K)
  • APPENDIX 4-3u: Plants_Herbaceous Dicot_Er-Il_CPY (XLSX)(1 pg, 545 K)
  • APPENDIX 4-3v: Plants_Herbaceous Dicot_Ip-Lu_CPY (XLSX)(1 pg, 509 K)
  • APPENDIX 4-3w: Plants_Herbaceous Dicot_Ly-Ph_CPY(XLSX)(1 pg, 509 K)
  • APPENDIX 4-3x: Plants_Herbaceous Dicot_Ph-Sc_CPY (XLSX)(1 pg, 509 K)
  • APPENDIX 4-3y: Plants_Herbaceous Dicot_Sc-St_CPY (XLSX)(1 pg, 507 K)
  • APPENDIX 4-3z: Plants_Herbaceous Dicot_St-Ye_CPY (XLSX)(1 pg, 490 K)
  • APPENDIX 4-3aa: Plants_Herbaceous Monocot_Ag-Pr_CPY (XLSX)(1 pg, 481 K)
  • APPENDIX 4-3bb: Plants_Herbaceous Monocot_Al-Pi_CPY (XLSX)(1 pg, 533 K)
  • APPENDIX 4-3cc: Plants_Herbaceous Monocot_Pl-Zi_CPY (XLSX)(1 pg, 530 K)
  • APPENDIX 4-3dd: Plants_Woody Dicot_Ab-Ce_CPY (XLSX)(1 pg, 528 K)
  • APPENDIX 4-3ee: Plants_Woody Dicot_Ch-Cy_CPY (XLSX)(1 pg, 505 K)
  • APPENDIX 4-3ff: Plants_Woody Dicot_Cy-Cy_CPY (XLSX)(1 pg, 508 K)
  • APPENDIX 4-3gg: Plants_Woody Dicot_Cy-Ga_CPY (XLSX)(1 pg, 508 K)
  • APPENDIX 4-3hh: Plants_Woody Dicot_Ga-La_CPY (XLSX)(1 pg, 508 K)
  • APPENDIX 4-3ii: Plants_Woody Dicot_La-My_CPY (XLSX)(1 pg, 508 K)
  • APPENDIX 4-3jj: Plants_Woody Dicot_My-Re_CPY (XLSX)(1 pg, 509 K)
  • APPENDIX 4-3kk: Plants_Woody Dicot_Re-Te_CPY (XLSX)(1 pg, 508 K)
  • APPENDIX 4-3ll: Plants_Woody Dicot_Te-Zi_CPY (XLSX)(1 pg, 484 K)
• APPENDIX 4-4: Risk Assessment for Cattle Ear Tag Use (DOCX)(89 pp, 285 K)
• APPENDIX 4-5: Analysis of Mosquito Adulticide Use (DOCX)(3 pp, 26 K)
  • APPENDIX 4-5 Supplemental Information 1: Mosquito Adulticide Animal Matrices (XLSX)(1 pg, 1.94 MB)
• APPENDIX 4-6: Analysis of Seed Treatment and Granular Uses (DOCX)(10 pp, 369 K)
• APPENDIX 4-7: Refined Risk Analyses for 14 Listed Birds Exposed to Chlorpyrifos (DOCX)(18 pp, 345 K)
  • APPENDIX 4-7 Supplemental Information 1: TIM/MCnest Metadata (DOCX)(48 pp, 112 K)