SPILLREMED (MARINE)®

TECHNICAL PRODUCT BULLETIN B-57
USEPA, OEM REGULATIONS IMPLEMENTATION DIVISION
LISTING DATE: JANUARY 8, 2007
“SPILLREMED (MARINE)^® (aka, AgroRemed, SpillRemed (Industrial), HydroRemed, VaporRemed)”

I. NAME, BRAND, OR TRADEMARK
SPILLREMED (MARINE)® (aka, AgroRemed, SpillRemed (Industrial), HydroRemed, VaporRemed) Type of Product: Bioremediation Agent (Biological Additive: Microbiological Culture)

II. NAME, ADDRESS, AND TELEPHONE NUMBER OF MANUFACTURER/CONTACT
Sarva Bio Remed, LLC 25 Marianne Drive, Suite ‘B’ York, PA 17406 Phone: (717) 779-0040 Phone: (877) 717-2782 Fax: (419) 710-5831 Email: sales@sarvabioremed.com Web Site: www.sarvabioremed.com Exit (Mr. Satya Ganti)

III. NAME, ADDRESS, AND TELEPHONE NUMBER OF PRIMARY DISTRIBUTORS
Sarva Bio Remed, LLC 25 Marianne Drive, Suite ‘B’ York, PA 17406 Phone: (717) 779-0040 Phone: (877) 717-2782 Fax: (419) 710-5831 Email: sales@sarvabioremed.com Web Site: www.sarvabioremed.com Exit (Mr. Satya Ganti) Edward R. Stephen Co. Inc. 10 Dunham Road Billerica, MA 01821 Phone: (978) 667-6399 E-mail: bohs@erstephen.com (Mr. Barry Ohs)

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION
1. Flammability: Non-flammable. 2. Ventilation: No special ventilation required. 3. Skin and eye contact; protective clothing; treatment in case of contact: On contact with product, wash with plenty of water and for eye contact consult your physician. Use of gloves and eye protection is recommended during field application to protect eyes from spray. 4.a. Maximum storage temperature: 100°F 4.b. Minimum storage temperature: 32°F 4.c. Optimum storage temperature range: Ambient 4.d. Temperatures of phase separations and chemical changes: <32°F and >100°F

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION

1. Flammability: Non-flammable.
2. Ventilation: No special ventilation required.
3. Skin and eye contact; protective clothing; treatment in case of contact: On contact with product, wash with plenty of water and for eye contact consult your physician. Use of gloves and eye protection is recommended during field application to protect eyes from spray.
4.a. Maximum storage temperature: 100°F
4.b. Minimum storage temperature: 32°F
4.c. Optimum storage temperature range: Ambient
4.d. Temperatures of phase separations and chemical changes: <32°F and >100°F

V. SHELF LIFE
The recommended shelf life is one year.

VI. RECOMMENDED APPLICATION PROCEDURE
1. Application Method: SPILLREMED (MARINE)® is a ready to use liquid product. It can be sprayed undiluted over the spill in open water conditions. 2. Concentration/Application Rate: SPILLREMED (MARINE)® is applied in a product to oil ratio of 1:10. Rate of application is one gallon per 10 minutes. 3. Conditions for Use: SPILLREMED (MARINE)® is a marine salt formulation and is not effective in fresh water conditions. Optimal effectiveness is in the salinity range of 10 to 35 ppt.

VI. RECOMMENDED APPLICATION PROCEDURE

1. Application Method:
SPILLREMED (MARINE)® is a ready to use liquid product. It can be sprayed undiluted over the spill in open water conditions.
2. Concentration/Application Rate:
SPILLREMED (MARINE)® is applied in a product to oil ratio of 1:10. Rate of application is one gallon per 10 minutes.
3. Conditions for Use:
SPILLREMED (MARINE)® is a marine salt formulation and is not effective in fresh water conditions. Optimal effectiveness is in the salinity range of 10 to 35 ppt.

VII. TOXICITY AND EFFECTIVENESS

a. Effectiveness:
Bioremediation Agent Effectiveness Test (40 CFR 300.900), Federal Register September 15, 1994:
Summary Data Table

DAYS	PRODUCT 3 Replct/Prod.	TOTAL MEAN ALKANE (ppm)	RED.% 28 Days	TOTAL MEAN AROMATICS (ppm)	RED.% 28 Days
0	CONTROL	48,963.31		13,467.59
	NUTRIENT	46,963.33		13,779.24
	SPILLREMED (MARINE)®	52,063.18		14,143.06
7	CONTROL	47,606.78		11,262.98
	NUTRIENT	40,853.28		11,329.01
	SPILLREMED (MARINE)®	43,236.67		11,076.99
28	CONTROL	45,467.28	7	12,560.37	7
	NUTRIENT	7,210.06	98	9,927.87	28
	SPILLREMED (MARINE)®	1,310.09	97	7,442.71	47

Results of Gravimetric Analysis:
Percentage (%) Decrease in Weight of Oil on Day 28
Control: 6.00%
Nutrient: 76%
Product: 85%

b. Toxicity:

MATERIAL TESTED	SPECIES	LC50 (ppm)
SPILLREMED (MARINE)®	Menidia beryllina Mysidopsis bahia	392.30 96-hr 343.80 48-hr
No. 2 Fuel Oil	Menidia beryllina Mysidopsis bahia	40.50 96-hr 17.40 48-hr
SPILLREMED (MARINE)® & No. 2 Fuel Oil (1:10)	Menidia beryllina Mysidopsis bahia	37.50 96-hr 22.40 48-hr
Reference Toxicant (SDS)	Menidia beryllina Mysidopsis bahia	8.00 96-hr 15.90 48-hr

VIII. MICROBIOLOGICAL ANALYSIS
1. Listing of all microorganisms by species and percentages in the composition: CONFIDENTIAL 2. Optimum pH, temperature, and salinity ranges for use of the additive: pH: 7.6 Temperature: 55°F Salinity: 30 ppt 3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity: pH: 6.5 – 6.9 Temperature: 40°F – 90°F Salinity: 10 ppt – 35 ppt 4. Special nutrient requirements: None 5. Test results regarding the determination of the presence of the following: Salmonella: Negative Fecal coliform: Negative Shigella: Negative Staphylococcus Coagulase positive: Negative Beta hemolytic Streptococci: Negative

VIII. MICROBIOLOGICAL ANALYSIS

1. Listing of all microorganisms by species and percentages in the composition:
CONFIDENTIAL
2. Optimum pH, temperature, and salinity ranges for use of the additive:
pH: 7.6
Temperature: 55°F
Salinity: 30 ppt
3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity:
pH: 6.5 – 6.9
Temperature: 40°F – 90°F
Salinity: 10 ppt – 35 ppt
4. Special nutrient requirements: None
5. Test results regarding the determination of the presence of the following:
Salmonella: Negative
Fecal coliform: Negative
Shigella: Negative
Staphylococcus Coagulase positive: Negative
Beta hemolytic Streptococci: Negative

IX. PHYSICAL PROPERTIES
NA

X. ANALYSIS FOR HEAVY METALS, CYANIDE, AND CHLORINATED HYDROCARBONS
NA