S-200C

TECHNICAL PRODUCT BULLETIN #B-56
USEPA, OEM REGULATIONS IMPLEMENTATION DIVISION
LISTING DATE: JULY 24, 2002
"S-200C"

I. NAME, BRAND, OR TRADEMARK
S-200C Type of Product: Bioremediation Agent (Nutrient Additive) (formerly S-200)

II. NAME, ADDRESS, AND TELEPHONE NUMBER OF MANUFACTURER/CONTACT
International Environmental Products, LLC Two Villanova Center 795 E. Lancaster Ave., Suite 280 Villanova, PA 19085 Phone: (610) 520-7665 Fax: (610) 520-7663 Email: info@iepusa.com (Mr. Jim Lynn)

III. NAME, ADDRESS, AND TELEPHONE NUMBER OF PRIMARY DISTRIBUTORS
International Environmental Products, LLC Two Villanova Center 795 E. Lancaster Ave., Suite 280 Villanova, PA 19085 Phone: (610) 520-7665 Fax: (610) 520-7663 Email: info@iepusa.com (Mr. Jim Lynn)

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION
1. Flammability: Non-flammable. 2. Ventilation: Ventilation of the work place is not necessary. 3. Skin and eye contact; protective clothing; treatment in case of contact: In case of contact with skin or eyes, wash with plenty of water. 4.a. Maximum storage temperature: 132ºF 4.b. Minimum storage temperature: 32ºF 4.c. Optimum storage temperature range: 55ºF - 85ºF 4.d. Temperatures of phase separations: <32ºF and >140ºF

IV. SPECIAL HANDLING AND WORKER PRECAUTIONS FOR STORAGE AND FIELD APPLICATION

1. Flammability:
Non-flammable.
2. Ventilation:
Ventilation of the work place is not necessary.
3. Skin and eye contact; protective clothing; treatment in case of contact:
In case of contact with skin or eyes, wash with plenty of water.
4.a. Maximum storage temperature: 132ºF
4.b. Minimum storage temperature: 32ºF
4.c. Optimum storage temperature range: 55ºF - 85ºF
4.d. Temperatures of phase separations: <32ºF and >140ºF

V. SHELF LIFE
The recommended shelf life of S-200C is 1½ years if kept in the unopened original container.

VI. RECOMMENDED APPLICATION PROCEDURE
1. Application Method: S-200C is a light amber liquid, and as such can be sprayed with traditional liquid spraying equipment, such as pressurized sprayers or backpack sprayers. 2. Concentration/Application Rate: The recommended application rate is approximately 10% by volume of the S-200C as compared to the hydrocarbon being remediated. In other words, use 1 pound of S-200C for every 10 pounds of hydrocarbon you are remediating. For applications on soil contaminated below 1 foot, the soil is turned and the material is applied. This allows air to circulate and thereby maintains an aerobic condition. On a surface application, the amount to use is approximately 0.1 to 1 pound of S-200C for every square yard of surface area. The amount sprayed is dependent on the amount of hydrocarbon on the surface you are spraying. To remediate a sheen on water, the same application ratio for a surface application is used. 3. Conditions for Use: S-200C is a bioremediation accelerator used for the remediation of hydrocarbon spills or leaks. It is a non-intrusive, cost effective remedy for the cleanup of these hydrocarbons. These hydrocarbons include but are not limited to gasoline, No. 2 to No. 6 diesel fuel, jet fuels, kerosene, lubricating oils, hydraulic oils, and crude oils.

VI. RECOMMENDED APPLICATION PROCEDURE

1. Application Method:
S-200C is a light amber liquid, and as such can be sprayed with traditional liquid spraying equipment, such as pressurized sprayers or backpack sprayers.
2. Concentration/Application Rate:
The recommended application rate is approximately 10% by volume of the S-200C as compared to the hydrocarbon being remediated. In other words, use 1 pound of S-200C for every 10 pounds of hydrocarbon you are remediating.
For applications on soil contaminated below 1 foot, the soil is turned and the material is applied. This allows air to circulate and thereby maintains an aerobic condition.
On a surface application, the amount to use is approximately 0.1 to 1 pound of S-200C for every square yard of surface area. The amount sprayed is dependent on the amount of hydrocarbon on the surface you are spraying.
To remediate a sheen on water, the same application ratio for a surface application is used.
3. Conditions for Use:
S-200C is a bioremediation accelerator used for the remediation of hydrocarbon spills or leaks. It is a non-intrusive, cost effective remedy for the cleanup of these hydrocarbons. These hydrocarbons include but are not limited to gasoline, No. 2 to No. 6 diesel fuel, jet fuels, kerosene, lubricating oils, hydraulic oils, and crude oils.

VII. TOXICITY AND EFFECTIVENESS

a. Toxicity

Material Tested	Species	LC50 (ppm)
S-200C	Menidia beryllina Mysidopsis bahia	39.69 96-hr 21.33 48-hr
No. 2 Fuel Oil	Menidia beryllina Mysidopsis bahia	35.36 96-hr 35.36 48-hr
S-200C & No. 2 Fuel Oil (1:10)	Menidia beryllina Mysidopsis bahia	25.33 96-hr 17.67 48-hr
Reference Toxicant (SDS)	Menidia beryllina Mysidopsis bahia	NA 96-hr 22.96 48-hr

b. Effectiveness:
Bioremediation Agent Effectiveness Test (40 CFR 300.900), Federal Register September 15, 1994:

Summary Data Table:

DAYS	PRODUCT 3 REPS/PROD	TOTAL MEAN ALKANES (ppm)	RED% 28 DAYS	TOTAL MEAN AROMATICS (ppm)	RED% 28 DAYS
0	CONTROL S-200C	27666 29333	0 0	5600 5666	0 0
7	CONTROL S-200C	21333 11000	0 0	4333 4400	0 0
28	CONTROL S-200C	18771 660	32 98	5597 5073	0.05 10.4

Results of Gravimetric Analysis:
Percentage (%) Decrease in Weight of Oil on Day 28

Control	Product (001)
-18.57%	27.82%

VIII. MICROBIOLOGICAL ANALYSIS
1. Listing of all microorganisms by species and percentages in the composition: S-200C is a nutrient bioremediation accelerator and does not contain bacterial cultures or amendments. However, with the addition of S-200C, the microbiological data shows a continued viability of microbial incubations over time. 2. Optimum pH, temperature, and salinity ranges for use of the additive: pH: 7.0 Temperature: 86ºF Salinity: Fresh to salt water 3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity: pH: 4.5 - 10.0 Temperature: 50ºF - 120ºF Salinity: 0% - 10% 4. Special nutrient requirements: NA 5. Test results regarding the determination of the presence of the following: Salmonella: Negative Fecal coliform: Negative Shigella: Negative Staphylococcus Coagulase positive: Negative Beta hemolytic Streptococci: Negative

VIII. MICROBIOLOGICAL ANALYSIS

1. Listing of all microorganisms by species and percentages in the composition:
S-200C is a nutrient bioremediation accelerator and does not contain bacterial cultures or amendments. However, with the addition of S-200C, the microbiological data shows a continued viability of microbial incubations over time.
2. Optimum pH, temperature, and salinity ranges for use of the additive:
pH: 7.0
Temperature: 86ºF
Salinity: Fresh to salt water
3. Minimum and maximum pH, temperature, and salinity levels below or above which the effectiveness of the additive is reduced to half its optimum capacity:
pH: 4.5 - 10.0
Temperature: 50ºF - 120ºF
Salinity: 0% - 10%
4. Special nutrient requirements:
NA
5. Test results regarding the determination of the presence of the following:
Salmonella: Negative
Fecal coliform: Negative
Shigella: Negative
Staphylococcus Coagulase positive: Negative
Beta hemolytic Streptococci: Negative

IX. PHYSICAL PROPERTIES
1. Flash Point: > 212ºF 2. Pour Point: 40ºF 3. Viscosity: 100cp @ 70ºF 4. Specific Gravity: 0.996 5. pH: 4.5 6. Surface Active Agents: NA 7. Solvents: NA 8. Additives: NA 9. Solubility in Water: Dispersable

X. ANALYSIS FOR HEAVY METALS, CYANIDE, AND CHLORINATED HYDROCARBONS
S-200C does not contain any heavy metals, cyanide, or chlorinated hydrocarbons.

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