Chronic Toxicity to Freshwater Organisms
This manual describes four- to seven-day methods for estimating the chronic toxicity of effluents and receiving waters to three species. The methods are approved under Clean Water Act section 304(h) and specified at 40 CFR 136.3, Table I–A.
The complete manual and individual method documents are provided. The individual methods are included in the manual.
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- Errata for Chronic Freshwater WET Methods Manual (4th edition, 2002) (PDF)(1 pg, 124 K, 2010)
- Short-term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms; 4th ed. (PDF)(350 pp, 5 MB, October 2002, 821-R-02-013)
- 1000.0: Fathead Minnow, Pimephales promelas, Larval Survival and Growth; Chronic Toxicity Test (PDF)(60 pp, 1 MB, October 2002)
- 1001.0: Fathead Minnow, Pimephales promelas, Larval Survival and Teratogenicity Test; Chronic Toxicity (PDF)(30 pp, 536 K, October 2002)
- 1002.0: Daphnid, Ceriodaphnia dubia, Survival and Reproduction Test; Chronic Toxicity (PDF)(57 pp, 911 K, October 2002)
- 1003.0: Green Alga, Selenastrum capricornutum, Growth Test; Chronic Toxicity (PDF)(35 pp, 614 K, October 2002)