Assays/Pathways

• Identify key toxicity pathways/assays (with a focus on human cells) and prioritize assays for use at National Center for Advancing Translational Sciences (NCATS)
• Identify methods for incorporating hepatic metabolism into in vitro assays
• Consider approaches for evaluating compound, pathway, and cell-to-cell interactions

Compounds

• Establish library of >10,000 compounds with known structures for testing at the NCATS
• Establish procedures for determining the identity, purity, and stability of each compound
• Future, establish a library of water soluble compounds for testing at the NCATS
• Future, establish a library of mixtures for testing at the NCATS

Bioinformatics Group

• Evaluate response within and across assays/endpoints
• Evaluate patterns of response and relationship to adverse health outcomes in experimental animals and humans
• Make all data publicly accessible (CEBS, PubChem, ACToR)

Targeted Testing Group

• Design studies to evaluate the relevance of prediction models and prioritization schemes developed from Tox21 data